重组Anti-ALDH7A1抗体[EP1934Y] (ab68192)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1934Y] to ALDH7A1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-ALDH7A1抗体[EP1934Y]
参阅全部 ALDH7A1 一抗 -
描述
兔单克隆抗体[EP1934Y] to ALDH7A1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human ALDH7A1 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Lysates from: HepG2 cells and mouse liver lysates. IHC-P: Human and mouse liver tissue.
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常规说明
The production method for this product has been changed from hybridoma to recombinant on 15th April 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1934Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68192于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Omim: 107323 Human
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- Unigene: 483239 Human
- Unigene: 30250 Mouse
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别名
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab68192 at 1/4000 (0.08 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab68192 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab68192 at 1/4000 (0.08 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab68192 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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All lanes : Anti-ALDH7A1 antibody [EP1934Y] (ab68192) at 1/10000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1. 10 seconds; Lane 2. 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab68192 被引用在 3 文献中.
- Etienne J et al. Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients. J Cachexia Sarcopenia Muscle 11:1047-1069 (2020). PubMed: 32157826
- Wijermars LGM et al. Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation. Oxid Med Cell Longev 2018:3704129 (2018). PubMed: 30671169
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924