This antibody is purified through a protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 58 kDa.
1/10 - 1/50.
Use at an assay dependent concentration. PubMed: 20966424
Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
ICC/IF image of ab63181 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63181 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Suryawanshi AR et al. Epididymosome Mediated Acquisition of MMSDH an Androgen Dependent and Developmentally Regulated Epididymal Sperm Protein. J Androl : (2011).
Read more (PubMed: 22207704) »
Suryawanshi AR et al. Differential Proteomics Leads to Identification of Domain Specific Epididymal Sperm Proteins. J Androl : (2010).
Read more (PubMed: 20966424) »