The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa.
Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
ab109582 (5µg/ml) shows staining of ALDH6A1 in paraffin embedded Human Liver following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows textured staining of the cytoplasm consistent with mitochondria.
Western blot - ALDH6A1 antibody (ab109582)
Anti-ALDH6A1 antibody (ab109582) at 0.1 µg/ml + Mouse Kidney lysate (in RIPA buffer) at 35 µg