Anti-ALDH6A1抗体(ab109576)

概述

  • 产品名称
    Anti-ALDH6A1抗体
    参阅全部 ALDH6A1 一抗
  • 描述
    山羊多克隆抗体to ALDH6A1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    SNKAGEYIFER

    by a Cysteine residue linker, corresponding to internal sequence amino acids 262-272 of Human ALDH6A1 (NP_005580.1).

  • 阳性对照
    • Human, Mouse and Rat Kidney lysates

性能

应用

Our Abpromise guarantee covers the use of ab109576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能
    Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
  • 疾病相关
    Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
  • 序列相似性
    Belongs to the aldehyde dehydrogenase family.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110038I05Rik antibody
    • AI314632 antibody
    • Aldehyde dehydrogenase 6 family member A1 antibody
    • Aldehyde dehydrogenase family 6 member A1 antibody
    • Aldehyde dehydrogenase family 6, subfamily A1 antibody
    • ALDH6A1 antibody
    • Malonate semialdehyde dehydrogenase antibody
    • Malonate-semialdehyde dehydrogenase [acylating] antibody
    • Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial antibody
    • Methylmalonate-semialdehyde dehydrogenase [acylating] antibody
    • MGC37325 antibody
    • MGC40271 antibody
    • Mitochondrial acylating methylmalonate semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • MMSA_HUMAN antibody
    • MMSADHA antibody
    • MMSDH antibody
    see all

图片

  • Anti-ALDH6A1 antibody (ab109576) at 0.1 µg/ml + Mouse kidney lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab109576 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse brain mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
20 µg
Specification
Mouse brain mitochondria
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
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提交于 Oct 06 2017

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