The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 50 kDa.
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
High levels in stomach, esophagus and lung; low level in the liver and kidney.
ab103897 (3.8µg/ml) staining of paraffin embedded Human Lung following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining strong staining of the respiratory epithelium of the tracheal wall.
Western blot - ALDH3A1 antibody (ab103895)
Anti-ALDH3A1 antibody (ab103895) at 0.01 µg/ml + Mouse Eye Lysate in RIPA buffer at 35 µg