重组
RabMAb

Anti-ALDH2抗体[EPR4493] (ab108306)

概述

  • 产品名称
    Anti-ALDH2抗体[EPR4493]
    参阅全部 ALDH2 一抗
  • 描述
    兔单克隆抗体[EPR4493] to ALDH2
  • 特异性
    The antibody immunogen has 82% homology with ALDH1B1 (P30837) and 77% with ALDH1A1 (P00352). BLAST results show for both proteins 6 aa in a row with 100% homology, therefore a cross-reactivity with these proteins is likely.
  • 经测试应用
    适用于: Flow Cyt, WB, IP, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.

  • 阳性对照
    • WB: HepG2 and A549 cell lysates and mouse liver and rat liver tissue lysates. IHC-P: Human colon tissue. Flow Cyt: HepG2 cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

应用

Our Abpromise guarantee covers the use of ab108306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC 1/100 - 1/250.

靶标

  • 通路
    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • 序列相似性
    Belongs to the aldehyde dehydrogenase family.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

图片

  • ab108306 at 1/50 dilution, staining ALDH2 in paraffin-embedded Human colon tissue
  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 56 kDa
  • Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ALDH2 (red) with ab108306 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 56 kDa

文献

This product has been referenced in:
  • Yasgar A  et al. A High-Content Assay Enables the Automated Screening and Identification of Small Molecules with Specific ALDH1A1-Inhibitory Activity. PLoS One 12:e0170937 (2017). Read more (PubMed: 28129349) »
  • Yu YH  et al. PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation. PLoS One 11:e0161993 (2016). WB, IP ; Mouse . Read more (PubMed: 27575855) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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