Anti-ALDH2抗体(ab115198)
Key features and details
- Rabbit polyclonal to ALDH2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALDH2抗体
参阅全部 ALDH2 一抗 -
描述
兔多克隆抗体to ALDH2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to amino acids from the N terminal region of ALDH2.
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阳性对照
- IHC-P: Human Liver tissue. Flow Cyt (Intra): HepG2 cells. WB: 293 cell lysate transiently transfected with the ALDH2 gene.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115198于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/50 - 1/100. Predicted molecular weight: 56 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50 - 1/100. Predicted molecular weight: 56 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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通路
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
序列相似性
Belongs to the aldehyde dehydrogenase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 217 Human
- Omim: 100650 Human
- SwissProt: P05091 Human
- Unigene: 604551 Human
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别名
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
图片
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ab115198 at 10 µg/ml staining ALDH2 Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Intracellular flow cytometric analysis of HepG2 cells, staining ALDH2 with ab115198 (bottom histogram), compared to a negative control cell (top histogram). A FITC-conjugated goat-anti-rabbit IgG was used as the secondary antibody.
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All lanes : Anti-ALDH2 antibody (ab115198) at 1/50 dilution
Lane 1 : 293 cell lysate, non-transfected
Lane 2 : 293 cell lysate, transiently transfected with ALDH2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 56 kDa
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab115198 尚未被引用在任何文献中。