Anti-ALDH2抗体[9A10AF10] (ab110311)

概述

  • 产品名称Anti-ALDH2抗体[9A10AF10]
    参阅全部 ALDH2 一抗
  • 描述
    小鼠单克隆抗体[9A10AF10] to ALDH2
  • 经测试应用适用于: ICC/IF, IP, In-Cell ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Tissue, cells or virus corresponding to ALDH2. Rat liver mitochondria.

  • 阳性对照
    • HepG2 cells; Human, Rat and Mouse liver and HepG2 cell lysates.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml. For 2 hours.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 5 µg/ml.

靶标

Anti-ALDH2 antibody [9A10AF10] 图像

  • Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
    Note: Target protein locates mainly in the mitochondrial matrix.
  • Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

Anti-ALDH2 antibody [9A10AF10] (ab110311)参考文献

ab110311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"