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Our Abpromise guarantee covers the use of ab96060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 57kDa; ALDH1A2
Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse testis tissue labeling ALDH1A2 with ab96060 at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"