Anti-ALDH1A1抗体- Neuronal Marker (ab24343)

概述

  • 产品名称
    Anti-ALDH1A1抗体- Neuronal Marker
    参阅全部 ALDH1A1 一抗
  • 描述
    兔多克隆抗体to ALDH1A1 - Neuronal Marker
  • 特异性
    May cross-react with mouse ALDH1A2 and ALDH1A3
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, IHC-FoFr, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length mouse ALDH1A1 protein (GenBank NM_013467) expressed as a fusion protein (Glutathione-S-Transferase) in E. coli.

  • 阳性对照
    • ALDH1A1 protein is a known mesencephalic dopaminergic marker.

性能

应用

Our Abpromise guarantee covers the use of ab24343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200.
WB Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.

靶标

  • 功能
    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • 通路
    Cofactor metabolism; retinol metabolism.
  • 序列相似性
    Belongs to the aldehyde dehydrogenase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

图片

  • ALDH1A1 antibody (ab24343) staining [A] tyrosine hydroxylase (TH; green) and [B] ALDH1A1 (red) -containing neurons in adult mouse brain sections by immunohistochemistry. [C] Overlap of TH and ALDH1A1-containing neurons. Pictures were taken with 20x objective. Sections were fixed in 4% formaldehyde for 30 min, rinsed with PBS and then incubated with blocking buffer (PBS, 10 % normal donkey serum; NDS) for 10 min. Sections were then incubated overnight at 4 degrees C with primary antibodies diluted in PBS containing 2% NDS. After additional rinsing in PBS, the coverslips were incubated in fluorescent-labeled secondary antibodies (Cy2-, Rhodamine Red-X-, or Cy5-labeled donkey IgG) in PBS with 2% NDS for 30 min at RT.

    ALDH1A1 antibody (ab24343) staining [A] tyrosine hydroxylase (TH; green) and [B] ALDH1A1 (red) -containing neurons in adult mouse brain sections by immunohistochemistry. [C] Overlap of TH and ALDH1A1-containing neurons. Pictures were taken with 20x objective. Sections were fixed in 4% formaldehyde for 30 min, rinsed with PBS and then incubated with blocking buffer (PBS, 10 % normal donkey serum; NDS) for 10 min. Sections were then incubated overnight at 4 degrees C with primary antibodies diluted in PBS containing 2% NDS. After additional rinsing in PBS, the coverslips were incubated in fluorescent-labeled secondary antibodies (Cy2-, Rhodamine Red-X-, or Cy5-labeled donkey IgG) in PBS with 2% NDS for 30 min at RT.

  • ab24343 staining ALDH1A1 - Neuronal Marker (green) in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 4 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI (blue).

    See Abreview

文献

This product has been referenced in:
  • Stott SR  et al. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease. PLoS One 12:e0171748 (2017). Read more (PubMed: 28182766) »
  • Rolland AS  et al. Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in vivo. Mol Ther Methods Clin Dev 3:16062 (2016). Read more (PubMed: 27689101) »

See all 14 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Mouse Tissue sections (Pancreatic cancer)
Specification
Pancreatic cancer
Permeabilization
Yes - PBS+Triton 0.05%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 25 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your new contact.

My colleague is out of office today. Checking the stock availability, it looks that we have only one batch of the ALDH1A1 antibody that was sent to you. We can offer you a new aliquot if you wish or a credit no...

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Thanks for the information.

We had orders from XXXXXXXXXXX in year 2008, the lot we sent was 369562. This lot is currently out of stock, so I am sorry we will not be able to send this lot. My suggestion would be to try a new vial of same lot h...

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Thank you very much for your email.

We generally recommend our customers trying 5-10 micron sections. I can recommend 8 micron thick sections than 16; the results will be much nicer. This could be reason why the antibody is not giving high int...

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Thank you very much for your email. I am sorry to hear to hear that you are having problems with this antibody.

We have not received any other complaint for this batch so the problem might be with the vial or with the protocol. In order to und...

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Thank you very much for your email. I am sorry to hear to hear that you are having problems with this antibody.

We have not received any other complaint for this batch so the problem might be with the vial or with the protocol. In order to un...

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Unfortunately the immunogen for ALDH1A1 is not available for sale.



At this timeonepossible way toobtain the protein is likely toa obtain theplasmid expressing mouse ALDH1A1 in E. coli or some other vector, and then isolate the prot...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (putative pancreatic cancer stem cell fraction)
Specification
putative pancreatic cancer stem cell fraction
Fixative
Formaldehyde
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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