Anti-ALDH1A1抗体[mAbcam61293] (ab61293)

概述

  • 产品名称Anti-ALDH1A1抗体[mAbcam61293]
    参阅全部 ALDH1A1 一抗
  • 描述
    小鼠单克隆抗体[mAbcam61293] to ALDH1A1
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human ALDH1A1.

    (Peptide available as ab92717.)

  • 阳性对照
    • This antibody gave a positive signal in human liver and kidney tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab61293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
    • 通路Cofactor metabolism; retinol metabolism.
    • 序列相似性Belongs to the aldehyde dehydrogenase family.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Anti-ALDH1A1 antibody [mAbcam61293] 图像

    • All lanes : Anti-ALDH1A1 antibody [mAbcam61293] (ab61293) at 10 µg/ml

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 42 kDa,74 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    Anti-ALDH1A1 antibody [mAbcam61293] (ab61293)参考文献

    ab61293 has not yet been referenced specifically in any publications.

    Product Wall

    As it is possible to offer custom- made conjugation, I would need to know what kind of fluorochrome you are interested in. I will then forward this information to our lab colleagues, who will let me know if we can help you in this case.

    Thank you very much for contacting us with your enquiry.

    We are not able to guarantee that our antibodies are sterile. Sodium azide is included in the buffer of ab61293 to limit microbial growth, however this can not guarantee sterility.
    Read More

    I am sorry for the delay in getting back to you in relation to the feasibility in producing the FITC conjugated version of the anti-ALDH1A1 antibody [mAbcam 61293] (ab61293).

    I am pleased to let you know on consideration of the work necessary ...

    Read More

    DISCOUNT CODE: ****

    Expiration date: March 6th, 2013
    Value: ***


    I am very pleased to hear you would like to accept our offer and test ab62193 in IHC-Fr. This code will give you *** off your next order before the expiratio...

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    Thank you for contacting us. Because we carry over 95,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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    Thank you for contacting us. Unfortunately we do not have an antibody that suits your needs. All of the ALDH1A1 antibodies have either been tested in mouse or have sufficient homology (greater than 80%) with the immunogen so that they are likely to rea...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Kidney)
    Specification Kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"