Alcohol Dehydrogenase Assay试剂盒(ab102533)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.01 mU/well
概述
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产品名称
Alcohol Dehydrogenase Assay试剂盒
参阅全部 Alcohol Dehydrogenase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media -
检测类型
Enzyme activity -
灵敏度
= 0.01 mU/well -
检测时间
0h 40m -
产品概述
Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Visit our FAQs page for tips and troubleshooting.
Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K787 Alcohol Dehydrogenase Activity Colorimetric Assay Kit. K787-100 is the same size as the 100 test size of ab102533.
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests ADH Positive Control 1 vial Assay Buffer XXXIV 1 x 25ml Developer VIII 1 vial Substrate XII 1 x 1ml NADH Standard I 1 vial -
研究领域
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相关性
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. -
细胞定位
Cytoplasmic -
别名
- ADH
- ADH alpha subunit
- ADH beta subunit
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab102533 被引用在 15 文献中.
- Hsueh CY et al. A positive feed-forward loop between Fusobacteriumnucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis. iScience 25:103829 (2022). PubMed: 35198889
- Fremdling P et al. A Preparative Mass Spectrometer to Deposit Intact Large Native Protein Complexes. ACS Nano 16:14443-14455 (2022). PubMed: 36037396
- Lee HY et al. Turmeric extract (Curcuma longa L.) regulates hepatic toxicity in a single ethanol binge rat model. Heliyon 8:e10737 (2022). PubMed: 36193527
- Yao D et al. Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading. Commun Biol 4:933 (2021). PubMed: 34413463
- Engevik M et al. Limosilactobacillus reuteri ATCC 6475 metabolites upregulate the serotonin transporter in the intestinal epithelium. Benef Microbes 12:583-599 (2021). PubMed: 34550056