重组
RabMAb

Anti-Alcohol Dehydrogenase抗体[EPR4440] (ab108197)

概述

  • 产品名称
    Anti-Alcohol Dehydrogenase抗体[EPR4440]
    参阅全部 Alcohol Dehydrogenase 一抗
  • 描述
    兔单克隆抗体[EPR4440] to Alcohol Dehydrogenase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Alcohol Dehydrogenase aa 200-300. The exact sequence is proprietary.

  • 阳性对照
    • WB: Human fetal liver, rat liver and mouse liver lysates IHC-P: Human liver tissue
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    图片

    • All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunohistochemical staining of Alcohol Dehydrogenase in paraffin embedded Human liver tissue, using ab108197 at a 1/100 dilution.

    文献

    ab108197 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (white adipose tissue)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    30 µg
    Specification
    white adipose tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. William Zhang

    Verified customer

    提交于 Mar 06 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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