Anti-Alcohol Dehydrogenase抗体[1E5] (ab8335)

概述

  • 产品名称Anti-Alcohol Dehydrogenase抗体[1E5]
    参阅全部 Alcohol Dehydrogenase 一抗
  • 描述
    小鼠单克隆抗体[1E5] to Alcohol Dehydrogenase
  • 特异性This antibody is against human Alcohol Dehydrogenase (ADH). This antibody reacts with ADH with Kd=10-10M This antibody does not influence oxidoreductase activity
  • 经测试应用适用于: IHC-P, ICCmore details
  • 种属反应性
    与反应: Human
    不与反应: Rat, Rabbit
  • 免疫原

    Purified alcohol dehydrogenase from human liver

  • 常规说明


    Immunocytochemical analysis of hepatocyte differentiation.

性能

应用

Our Abpromise guarantee covers the use of ab8335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.

靶标

  • 序列相似性Belongs to the zinc-containing alcohol dehydrogenase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADH alpha subunit antibody
    • ADH antibody
    • ADH1 antibody
    • ADH1A antibody
    • ADH1A_HUMAN antibody
    • Alcohol dehydrogenase 1 antibody
    • Alcohol dehydrogenase 1A antibody
    • Alcohol dehydrogenase subunit alpha antibody
    • Aldehyde reductase antibody
    see all

Anti-Alcohol Dehydrogenase antibody [1E5] 图像

  • Ab8335 staining human normal liver. Staining is localised to the cytoplasmic compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Alcohol Dehydrogenase antibody [1E5] (ab8335)参考文献

ab8335 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can confirm that the antibody was raised to ADH1A but unfortunately we have not tested it with other isoenzymes. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Unfortunately we have not tested the subtype specificity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"