Albumin猪ELISA试剂盒(ab108794)

概述

  • 产品名称
    Albumin猪ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.6%
    批次间
    样品 n Mean SD CV%
    Overall 10.6%
  • 样品类型
    Cell culture supernatant, Urine
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 1.1 ng/ml
  • 范围
    1.563 ng/ml - 100 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Pig
  • 产品概述

    Abcam’s Albumin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in urine and cell culture supernatants.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab108794

实验方案

文献

ab108794 has not yet been referenced specifically in any publications.

客户评价及客户问答

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