Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide within residues: VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS, corresponding to N terminal amino acids 144-193 of Human Alas1 (NP_000679).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Information by UniProt
5 aminolevulinate synthase antibody
5 aminolevulinate synthase nonspecific mitochondrial antibody
5 aminolevulinic acid synthase antibody
Western blot - Alas1 antibody (ab84962)
Anti-Alas1 antibody (ab84962) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size:
Observed band size:
65 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
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