Anti-AlaRS抗体- N-terminal (ab71289)

概述

  • 产品名称
    Anti-AlaRS抗体- N-terminal
    参阅全部 AlaRS 一抗
  • 描述
    兔多克隆抗体to AlaRS - N-terminal
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 阳性对照
    • K562 cell line lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    ab71289 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
IHC-P 1/10 - 1/50.

靶标

  • 功能
    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
  • 疾病相关
    Charcot-Marie-Tooth disease 2N
  • 序列相似性
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 结构域
    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
    The C-terminal C-Ala domain (residues 756 to 968), along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. The human domain can be used in vitro to replace the corresponding domain in E.coli.
  • 翻译后修饰
    ISGylated.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AARS antibody
    • AI316495 antibody
    • Alanine tRNA ligase 1, cytoplasmic antibody
    • Alanine tRNA ligase antibody
    • Alanine tRNA ligase cytoplasmic antibody
    • Alanine--tRNA ligase antibody
    • Alanyl tRNA synthetase antibody
    • Alanyl tRNA synthetase cytoplasmic antibody
    • Alanyl-tRNA synthetase antibody
    • AlaRS antibody
    • C76919 antibody
    • CMT2N antibody
    • cytoplasmic antibody
    • EC 6.1.1.7 antibody
    • MGC37368 antibody
    • Renal carcinoma antigen NY REN 42 antibody
    • Renal carcinoma antigen NY-REN-42 antibody
    • SYAC_HUMAN antibody
    see all

图片

  • Anti-AlaRS antibody - N-terminal (ab71289) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 107 kDa
    Observed band size : 107 kDa
  • ab71289 at 1/50 dilution staining AlaRS N - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

文献

ab71289 has not yet been referenced specifically in any publications.

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