Anti-Alanine Transaminase抗体(ab34832)

概述

  • 产品名称Anti-Alanine Transaminase抗体
    参阅全部 Alanine Transaminase 一抗
  • 描述
    羊多克隆抗体to Alanine Transaminase
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other tissues may occur but have not been specifically determined.
  • 经测试应用适用于: Dot blot, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Pig
  • 免疫原

    Full length protein from Pig heart.

性能

应用

Our Abpromise guarantee covers the use of ab34832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
WB
ELISA
IP
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/5000. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.

    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
    • 组织特异性Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
    • 通路Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
    • 序列相似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AAT1 antibody
      • Alanine aminotransferase 1 antibody
      • Alanine aminotransferase antibody
      • ALAT1_HUMAN antibody
      • ALT1 antibody
      • Glutamate pyruvate transaminase 1 antibody
      • Glutamic alanine transaminase 1 antibody
      • Glutamic pyruvate transaminase (alanine aminotransferase) antibody
      • Glutamic pyruvic transaminase 1 antibody
      • Glutamic--alanine transaminase 1 antibody
      • Glutamic--pyruvic transaminase 1 antibody
      • GPT 1 antibody
      • gpt antibody
      • GPT1 antibody
      see all

    Anti-Alanine Transaminase antibody (ab34832)参考文献

    ab34832 has not yet been referenced specifically in any publications.

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