特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other
tissues may occur but have not been specifically determined.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000. This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Suitable for most immunological methods requiring high titer and specificity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
组织特异性Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
通路Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
序列相似性Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.