概述

  • 产品名称Anti-AKT3抗体
    参阅全部 AKT3 一抗
  • 描述
    小鼠单克隆抗体to AKT3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human AKT3 aa 100-189.
    Sequence:

    EAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFD YLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDE


    Database link: Q9Y243

  • 阳性对照
    • AKT3 recombinant protein, HeLa cell lysate, Raw 264.7 cell lysate, MCF7 cell lysate and NIH/3T3 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab89430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • 组织特异性In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 结构域Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • 翻译后修饰Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • 细胞定位Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Akt3 antibody
    • AKT3 kinase antibody
    • AKT3_HUMAN antibody
    • DKFZp434N0250 antibody
    • MPPH antibody
    • PKB gamma antibody
    • PKBG antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein Kinase AKT3 antibody
    • Protein kinase B gamma antibody
    • RAC gamma antibody
    • RAC gamma serine/threonine protein kinase antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACPK Gamma antibody
    • Serine threonine protein kinase Akt 3 antibody
    • Serine threonine protein kinase Akt3 antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
    see all

Anti-AKT3 antibody 图像

  • Anti-AKT3 antibody (ab89430) at 5 µg/ml + AKT3 recombinant protein fragment at 0.2 µg

    Predicted band size : 56 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-AKT3 antibody (ab89430) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-AKT3 antibody (ab89430) at 5 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-AKT3 antibody (ab89430) at 5 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Detection limit for recombinant AKT3 is approximately 0.1ng/ml as a capture antibody.
  • Anti-AKT3 antibody (ab89430) at 5 µg/ml + MCF7 cell lysate at 50 µg

    Predicted band size : 56 kDa

Anti-AKT3 antibody (ab89430)参考文献

ab89430 has not yet been referenced specifically in any publications.

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