概述

  • 产品名称
  • 描述
    山羊多克隆抗体to AKT3
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human AKT3 aa 119-133 (internal sequence).
    Sequence:

    CSPTSQIDNIGEEEM


    Database link: Q9Y243

  • 阳性对照
    • Human heart and prostate tissues; HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab112002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 56 kDa. for 1 hr.
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/16000.

靶标

  • 功能
    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • 组织特异性
    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 结构域
    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • 翻译后修饰
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • 细胞定位
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Akt3 antibody
    • AKT3 kinase antibody
    • AKT3_HUMAN antibody
    • DKFZp434N0250 antibody
    • MPPH antibody
    • PKB gamma antibody
    • PKBG antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein Kinase AKT3 antibody
    • Protein kinase B gamma antibody
    • RAC gamma antibody
    • RAC gamma serine/threonine protein kinase antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACPK Gamma antibody
    • Serine threonine protein kinase Akt 3 antibody
    • Serine threonine protein kinase Akt3 antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
    see all

图片

  • ab112002, at µg/ml, staining AKT3 in formalin-fixed, paraffin-embedded Human Heart tissue by Immunohistochemistry.
  • ab112002, at µg/ml, staining AKT3 in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • Anti-AKT3 antibody (ab112002) at 1 µg/ml + HepG2 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 56 kDa

文献

This product has been referenced in:
  • Zhou K  et al. MicroRNA-145 inhibits the activation of the mTOR signaling pathway to suppress the proliferation and invasion of invasive pituitary adenoma cells by targeting AKT3 in vivo and in vitro. Onco Targets Ther 10:1625-1635 (2017). Read more (PubMed: 28352194) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.

We have not specifically tested this antibody for cross-reactivity with AKT1 or AKT2, but the immunogen sequence is only 53% homologous with AKT1 and 13% homologous with AKT2. We would not predict this antibody to...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册