概述

  • 产品名称Anti-AKT3 + AKT1抗体[E45]
    参阅全部 AKT3+AKT1 一抗
  • 描述
    兔单克隆抗体[E45] to AKT3 + AKT1
  • 特异性ab32038 detects Akt1 and Akt3.
  • 经测试应用适用于: WB, IHC-P, ICC, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • 阳性对照
    • Squamous lung carcinoma
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. PubMed: 19679212
IHC-P 1/250 - 1/1000.
ICC 1/1000.
IP 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    Anti-AKT3 + AKT1 antibody [E45] 图像

    • All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

      Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
      Lane 2 : Empty vector transfected 293T cell lysate
      Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
      Lane 4 : Empty vector transfected 293T cell lysate
      Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
      Lane 6 : Empty vector transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Exposure time : 1 second

      Blocking/Diluting buffer: 5% NFDM/TBST.

      Observed band: 82kDa, 56kDa.

      82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

    • Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma

    Anti-AKT3 + AKT1 antibody [E45] (ab32038)参考文献

    This product has been referenced in:
    • Chen YC  et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep 6:18815 (2016). WB ; Human . Read more (PubMed: 26739898) »
    • Zhang Z  et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1. Mol Med Rep 11:857-64 (2015). WB ; Human . Read more (PubMed: 25369801) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"