概述

  • 产品名称Anti-AKT2抗体
    参阅全部 AKT2 一抗
  • 描述
    兔多克隆抗体to AKT2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human AKT2.

    (Peptide available as ab87440.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; HEK293.

性能

应用

Our Abpromise guarantee covers the use of ab78666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能General protein kinase capable of phosphorylating several known proteins.
  • 组织特异性Expressed in all human cell types so far analyzed.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Akt2 antibody
    • AKT2_HUMAN antibody
    • HIHGHH antibody
    • murine thymoma viral (v-akt) homolog-2 antibody
    • PKB antibody
    • PKB beta antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase B beta antibody
    • rac protein kinase beta antibody
    • RAC-BETA antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-PK-beta antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    see all

Anti-AKT2 antibody 图像

  • All lanes : Anti-AKT2 antibody (ab78666) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

Anti-AKT2 antibody (ab78666)参考文献

ab78666 has not yet been referenced specifically in any publications.

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