Anti-AKR1C1/AKR1C2抗体(ab96087)

概述

  • 产品名称Anti-AKR1C1/AKR1C2抗体
    参阅全部 AKR1C1/AKR1C2 一抗
  • 描述
    兔多克隆抗体to AKR1C1/AKR1C2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 22-218 of Human AKR1C1 (NP_001344).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates. IHC-P: MDA-MB-468 xenograft ICC/IF: methanol fixed SKNSH cells.

性能

应用

Our Abpromise guarantee covers the use of ab96087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Anti-AKR1C1/AKR1C2 antibody 图像

  • Anti-AKR1C1/AKR1C2 antibody (ab96087) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 37 kDa
  • Immunohistochemical analysis of AKR1C1 in paraffin embedded MDA-MB-468 xenograft, using ab96087 at a 1/500 dilution.
  • Anti-AKR1C1/AKR1C2 antibody (ab96087) at 1/1000 dilution + Recombinant Human AKR1C1 protein (ab93730) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • ICC/IF image of ab96087 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96087 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-AKR1C1/AKR1C2 antibody (ab96087)参考文献

ab96087 has not yet been referenced specifically in any publications.

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Thank you for contacting us. The immunogen for ab96087 shows 97% identity with human AKR1C2, so it will likely cross-react. This has not been confirmed experimentally. I hope this information is helpful to you. Please do not hesitate to contact ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"