Synthetic peptide from the center region of human AKR1B1 conjugated to KLH
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71405 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Belongs to the aldo/keto reductase family.
Information by UniProt
AKR1B 1 antibody
Western blot - Anti-AKR1B1 antibody (ab71405)
Anti-AKR1B1 antibody (ab71405) at 1/60 dilution + Jurkat cell line lysates at 35 µg
Predicted band size: 36 kDa Observed band size: 36 kDa Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B1 antibody (ab71405)
ab71405 at 1/50 dilution staining AKR1B1 in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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