antigen sequence: LEWKQNVEKK GHGRINCGRF SIVLHEKAPH SDSTPNSDTG HSFYSDSGTE MQSNKCQDCG TKIPTSRRAC RKEPTKEFHY, corresponding to amino acids of Human AKNAD1.
- Human lymph node tissue; RT 4, U 251 MG, Human liver and tonsil lysates; A431 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the AKNA family.
Information by UniProt
- AKNA domain containing 1 antibody
- AKNAD1 antibody
- AKND1_HUMAN antibody
Western blot - Anti-AKNAD1 antibody (ab122104)
All lanes : Anti-AKNAD1 antibody (ab122104) at 1/250 dilution
Lane 1 : RT cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKNAD1 antibody (ab122104)
ab122104, at 1/50 dilution, staining AKNAD1 in paraffin embedded Human lymph node tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-AKNAD1 antibody (ab122104)
ab122104 at 4 µg/ml, staining AKNAD1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"