概述

  • 产品名称Anti-AKIP抗体
    参阅全部 AKIP 一抗
  • 描述
    小鼠多克隆抗体to AKIP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length AKIP protein (Human)

  • 阳性对照
    • WB: AKIP transfected 293T cell lysate. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab67343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.

靶标

  • 功能May act as a negative regulator of Aurora-A kinase, by down-regulation through proteasome-dependent degradation.
  • 组织特异性Ubiquitously expressed and especially highly expressed in heart, skeletal muscle and testis.
  • 细胞定位Mitochondrion. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 28S ribosomal protein S38 antibody
    • AIP antibody
    • AKIP_HUMAN antibody
    • AURKA interacting protein antibody
    • AURKA-interacting protein antibody
    • AURKAIP 1 antibody
    • Aurkaip1 antibody
    • Aurora A kinase interacting protein antibody
    • Aurora kinase A interacting protein 1 antibody
    • Aurora kinase A-interacting protein antibody
    • FLJ20608 antibody
    • mitochondrial antibody
    • MRP-S38 antibody
    see all

Anti-AKIP antibody 图像

  • All lanes : Anti-AKIP antibody (ab67343) at 1/500 dilution

    Lane 1 : AKIP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescent staining of AKIP in HeLa cells using purified ab67343 at 10 ug/ml.

Anti-AKIP antibody (ab67343)参考文献

ab67343 has not yet been referenced specifically in any publications.

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