Anti-AKAP9抗体(ab31307)

概述

  • 产品名称Anti-AKAP9抗体
    参阅全部 AKAP9 一抗
  • 描述
    山羊多克隆抗体to AKAP9
  • 特异性This antibody is expected to recognise three of the four reported isoforms (NP_005742; NP_671695; NP_671714) and MAY also recognise NP_671700 whose last 4 aa differ from the others.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    (C)SGSTTQFHAGMR

    , corresponding to C terminal amino acids 3899-3910 of Human AKAP9

  • 阳性对照
    • HepG2 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 454 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
    • 组织特异性Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas.
    • 疾病相关Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
    • 结构域RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A kinase (PRKA) anchor protein (yotiao) 9 antibody
      • A kinase (PRKA) anchor protein 9 antibody
      • A kinase anchor protein 350kDa antibody
      • A kinase anchor protein 450 kDa antibody
      • A kinase anchor protein 9 antibody
      • A kinase anchoring protein 350 antibody
      • A kinase anchoring protein 450 antibody
      • A-kinase anchor protein 350 kDa antibody
      • A-kinase anchor protein 450 kDa antibody
      • A-kinase anchor protein 9 antibody
      • AKAP 120-like protein antibody
      • AKAP 350 antibody
      • AKAP 450 antibody
      • AKAP 9 antibody
      • AKAP-9 antibody
      • AKAP120 like protein antibody
      • AKAP350 antibody
      • AKAP450 antibody
      • AKAP9 antibody
      • AKAP9_HUMAN antibody
      • Centrosome and Golgi localized PKN associated protein antibody
      • Centrosome and golgi localized protein antibody
      • Centrosome- and Golgi-localized PKN-associated protein antibody
      • CG NAP antibody
      • CG-NAP antibody
      • hgAKAP 350 antibody
      • hgAKAP350 antibody
      • Hyperion antibody
      • KIAA0803 antibody
      • Kinase N associated protein antibody
      • MU RMS 40.16A antibody
      • PRKA 9 antibody
      • PRKA9 antibody
      • Protein hyperion antibody
      • Protein kinase A anchoring protein 9 antibody
      • Protein kinase A-anchoring protein 9 antibody
      • Protein yotiao antibody
      • Yotiao antibody
      see all

    Anti-AKAP9 antibody (ab31307)参考文献

    ab31307 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - nuclear (CH12 mouse B cell line)
    Loading amount 50 µg
    Specification CH12 mouse B cell line
    Treatment IL4, CD40, TGFbeta for 48 hours
    Gel Running Conditions Reduced Denaturing (6% SDS-PAGE)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Jan 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"