概述

  • 产品名称Anti-AKAP8L抗体
    参阅全部 AKAP8L 一抗
  • 描述
    兔多克隆抗体to AKAP8L
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GAVSPPPPPP PEEEEEGAVP LLGGALQRQI RGIPGLDVED DEEGGGGAP, corresponding to C terminal amino acids 598-646 of Human AKAP8L

  • 阳性对照
    • Jurkat cell lysate, human lung and human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. It does not seem to be implicated in the binding of regulatory subunit II of PKA. It may be involved in nuclear envelope breakdown and chromatin condensation. It may regulate the initiation phase of DNA replication when associated with TMPO-beta.
  • 细胞定位Nuclear matrix. Cytoplasm. Note=Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix.
  • 数据库链接
  • 别名
    • A kinase (PRKA) anchor protein 8 like antibody
    • A kinase anchor protein 8 like antibody
    • AKAP 8L antibody
    • AKAP8 like protein antibody
    • DKFZp434L0650 antibody
    • HA95 antibody
    • HAP95 antibody
    • Helicase A binding protein 95 antibody
    • Homologous to AKAP95 protein antibody
    • NAKAP antibody
    • NAKAP95 antibody
    • Neighbor of A kinase anchoring protein 95 antibody
    • Neighbor of AKAP95 antibody
    see all

Anti-AKAP8L antibody 图像

  • Anti-AKAP8L antibody (ab51342) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling AKAP8L with ab51342 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling AKAP8L with abAKAP8L at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

Anti-AKAP8L antibody (ab51342)参考文献

This product has been referenced in:

See 1 Publication for this product

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