Anti-AKAP 95抗体(ab72196)
Key features and details
- Rabbit polyclonal to AKAP 95
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-AKAP 95抗体
参阅全部 AKAP 95 一抗 -
描述
兔多克隆抗体to AKAP 95 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to Human AKAP 95 aa 642-692 (C terminal).
Database link: NP_005849.1 -
阳性对照
- HeLa and 293T whole cell lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). -
组织特异性
Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the AKAP95 family.
Contains 2 C2H2 AKAP95-type zinc fingers. -
细胞定位
Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 10270 Human
- Entrez Gene: 56399 Mouse
- Omim: 604692 Human
- SwissProt: O43823 Human
- SwissProt: Q9DBR0 Mouse
- Unigene: 594496 Human
- Unigene: 328945 Mouse
-
别名
- A kinase (PRKA) anchor protein 8 antibody
- A kinase anchor protein 8 antibody
- A kinase anchor protein 95 antibody
see all
图片
-
All lanes : Anti-AKAP 95 antibody (ab72196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP 95 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
-
Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
Lane 2 represents a Control IgG.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab72196 被引用在 2 文献中.
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Liu W et al. Roles of Cx43 and AKAP95 in ovarian cancer tissues in G1/S phase. Int J Clin Exp Pathol 8:14315-24 (2015). Human . PubMed: 26823747