参阅全部 AKAP8 一抗
- 特异性ab65039 detects endogenous levels of total AKAP8 protein.
ELISA, ICC/IF, IHC-P, WBmore details
Synthetic peptide derived from an internal sequence of human AKAP8.
- IHC-P: Human heart tissue.
WB: Extracts from mouse heart, Jurkat and HUVEC cells.
- 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明ab65039 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000.
||1/50 - 1/100.
||1/500 - 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
- 功能Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
- 组织特异性Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
- 序列相似性Belongs to the AKAP95 family.
Contains 2 C2H2 AKAP95-type zinc fingers.
- 细胞定位Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
Information by UniProt
- A kinase (PRKA) anchor protein 8 antibody
- A kinase anchor protein 8 antibody
- A kinase anchor protein 95 antibody
Anti-AKAP8 antibody 图像
Immunocytochemistry/ Immunofluorescence - AKAP8 antibody (ab65039)
ab65039, at a 1/500 dilution, staining AKAP8 in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKAP8 antibody (ab65039)
ab65039, at a 1/50 dilution, staining AKAP8 in paraffin embedded human heart tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
Western blot - AKAP8 antibody (ab65039)
All lanes : Anti-AKAP8 antibody (ab65039) at 1/500 dilution
Lane 1 : Mouse heart cell extract
Lane 2 : Jurkat cell extract
Lane 3 : Jurkat cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 76 kDa
Observed band size : 76 kDa
Anti-AKAP8 antibody (ab65039)参考文献
has not yet been referenced specifically in any publications.
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