Mouse, Rat, Dog
, corresponding to N terminal amino acids 2 - 14 of Human AKAP8.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: 1/32000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 96 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
Belongs to the AKAP95 family.
Contains 2 C2H2 AKAP95-type zinc fingers.
Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
Information by UniProt
- A kinase (PRKA) anchor protein 8 antibody
- A kinase anchor protein 8 antibody
- A kinase anchor protein 95 antibody
Western blot - AKAP8 antibody (ab23930)
Anti-AKAP8 antibody (ab23930) at 1 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane). Predicted band size:
96 kDaObserved band size:
100 kDa (why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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