概述

  • 产品名称Anti-AKAP5抗体
    参阅全部 AKAP5 一抗
  • 描述
    兔多克隆抗体to AKAP5
  • 特异性ab64851 detects endogenous levels of total AKAP5 protein.
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human AKAP5.

  • 阳性对照
    • Extracts from HepG2 cells, HeLa cells and COLO205 cells. HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab64851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

靶标

  • 功能May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
  • 组织特异性Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
  • 序列相似性Contains 1 AKAP domain.
  • 结构域RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • 细胞定位Membrane. Associated with particulate fractions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A kinase (PRKA) anchor protein 5 antibody
    • A kinase anchor protein 5 antibody
    • A kinase anchor protein 79kDa antibody
    • A kinase anchoring protein 75/79 antibody
    • A-kinase anchor protein 5 antibody
    • A-kinase anchor protein 79 kDa antibody
    • AKAP 5 antibody
    • AKAP 75 antibody
    • AKAP 79 antibody
    • AKAP-5 antibody
    • Akap5 antibody
    • AKAP5_HUMAN antibody
    • AKAP75 antibody
    • AKAP79 antibody
    • cAMP dependent protein kinase regulatory subunit II high affinity binding protein antibody
    • cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein antibody
    • H21 antibody
    see all

Anti-AKAP5 antibody 图像

  • All lanes : Anti-AKAP5 antibody (ab64851) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HeLa cells
    Lane 3 : Extracts from COLO205 cells
    Lane 4 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells using AKAP5 antibody (ab64851), at 1/500 dilution, in the absence (left) or presence (right) of immunising peptide.

Anti-AKAP5 antibody (ab64851)参考文献

ab64851 has not yet been referenced specifically in any publications.

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