重组Anti-AKAP3抗体[EPR11261] (ab170856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11261] to AKAP3
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-AKAP3抗体[EPR11261]
参阅全部 AKAP3 一抗 -
描述
兔单克隆抗体[EPR11261] to AKAP3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human AKAP3. The exact sequence is proprietary.
Database link: O75969 -
阳性对照
- Y79, HuT-78, HeLa, 293T, NIH 3T3, NCI-H460 and Jurkat whole cell lysate (ab7899); HeLa cells; Permeabilized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11261 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170856于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 95 kDa.
|
|
ICC/IF |
1/250 - 1/500.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 95 kDa. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. -
组织特异性
Testis specific; only expressed in spermatids. -
序列相似性
Belongs to the AKAP110 family. -
结构域
RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
翻译后修饰
Phosphorylated on tyrosine residues. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment. - Information by UniProt
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数据库链接
- Entrez Gene: 10566 Human
- Entrez Gene: 11642 Mouse
- Entrez Gene: 312720 Rat
- Omim: 604689 Human
- SwissProt: O75969 Human
- SwissProt: O88987 Mouse
- Unigene: 98397 Human
- Unigene: 87748 Mouse
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别名
- A kinase (PRKA) anchor protein 3 antibody
- A-kinase anchor protein 110 kDa antibody
- A-kinase anchor protein 3 antibody
see all
图片
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All lanes : Anti-AKAP3 antibody [EPR11261] (ab170856) at 1/1000 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 5 : NIH/3T3 cell lysate
Lane 6 : NCI-H460 cell lysate
Lane 7 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 95 kDa -
Immunofluorescent analysis of HeLa cells labeling AKAP3 with ab170856 at 1/250 dilution (red). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling AKAP3 with ab170856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab170856 被引用在 2 文献中.
- Xu Y et al. Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification. Sci Rep 6:36515 (2016). ICC/IF, WB ; Human . PubMed: 27857155
- Huang YL et al. Spermatogenesis-associated proteins at different developmental stages of buffalo testicular seminiferous tubules identified by comparative proteomic analysis. Proteomics 16:2005-18 (2016). PubMed: 27173832