Anti-AKAP3抗体(ab19046)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to AKAP3
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性

    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    TPLQLLDWLMVNL

    , corresponding to C terminal amino acids 841-853 of Human AKAP3 .

  • 阳性对照
    • Human Testis lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa).
ELISA 1/32000.

靶标

  • 功能
    May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
  • 组织特异性
    Testis specific; only expressed in spermatids.
  • 序列相似性
    Belongs to the AKAP110 family.
  • 结构域
    RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • 翻译后修饰
    Phosphorylated on tyrosine residues.
  • 细胞定位
    Cytoplasmic vesicle > secretory vesicle > acrosome. Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A kinase (PRKA) anchor protein 3 antibody
    • A-kinase anchor protein 110 kDa antibody
    • A-kinase anchor protein 3 antibody
    • AKAP 110 antibody
    • AKAP-3 antibody
    • AKAP110 antibody
    • Akap3 antibody
    • AKAP3_HUMAN antibody
    • Cancer/testis antigen 82 antibody
    • CT82 antibody
    • Fibrous sheath protein of 95 kDa antibody
    • Fibrousheathin 1 antibody
    • Fibrousheathin I antibody
    • Fibrousheathin-1 antibody
    • FSP95 antibody
    • PRKA3 antibody
    • protein kinase A binding protein AKAP 110 antibody
    • Protein kinase A-anchoring protein 3 antibody
    • SOB 1 antibody
    • SOB1 antibody
    • Sperm oocyte binding protein 1 antibody
    • Sperm oocyte-binding protein antibody
    see all

Anti-AKAP3 antibody 图像



  • Predicted band size : 95 kDa


    ab19046 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) approx. 90kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

实验方案

Anti-AKAP3 antibody (ab19046)参考文献

ab19046 has not yet been referenced specifically in any publications.

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Thank you for contacting us with your question.

We have not tested either of these antibodies in immunofluorescent staining yet, so we do not have an optimized dilution factor for them. I would recommend titrating the antibodies from 1-5 ug/m...

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