概述

  • 产品名称Anti-AKAP13抗体
    参阅全部 AKAP13 一抗
  • 描述
    兔多克隆抗体to AKAP13
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 2754 and 2804 of Human AKAP13 (NP_009131.2)

  • 阳性对照
    • HeLa whole cell lysate
  • 常规说明Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

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应用

Our Abpromise guarantee covers the use of ab99377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 308 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.
  • 组织特异性Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • 结构域Both the DH and PH domains are required for transforming activity.
  • 细胞定位Cytoplasm; Cytoplasm. Nucleus and Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A kinase (PRKA) anchor protein 13 antibody
    • A kinase anchor protein 13 antibody
    • A kinase anchoring protein antibody
    • A-kinase anchor protein 13 antibody
    • AKAP 13 antibody
    • AKAP Lbc antibody
    • AKAP-13 antibody
    • AKAP-Lbc antibody
    • AKAP13 antibody
    • AKP13_HUMAN antibody
    • ARHGEF13 antibody
    • Breast cancer nuclear receptor binding auxiliary protein antibody
    • Breast cancer nuclear receptor-binding auxiliary protein antibody
    • BRX antibody
    • c lbc antibody
    • FLJ11952 antibody
    • FLJ43341 antibody
    • Guanine nucleotide exchange factor Lbc antibody
    • HA 3 antibody
    • HA3 antibody
    • Ht 31 antibody
    • Ht31 antibody
    • Human thyroid anchoring protein 31 antibody
    • Human thyroid-anchoring protein 31 antibody
    • LBC antibody
    • LBC oncogene antibody
    • Lymphoid blast crisis oncogene antibody
    • Non oncogenic Rho GTPase specific GTP exchange factor antibody
    • Non-oncogenic Rho GTPase-specific GTP exchange factor antibody
    • p47 antibody
    • Protein kinase A anchoring protein 13 antibody
    • Protein kinase A-anchoring protein 13 antibody
    • PROTO LB antibody
    • PROTO LBC antibody
    see all

Anti-AKAP13 antibody 图像

  • All lanes : Anti-AKAP13 antibody (ab99377) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 308 kDa


    Exposure time : 10 seconds
  • Lane 1 : Detection of Human AKAP13 by Immunoprecipitation from HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded) using 3µg/mg lysate ab99377 lane 2 : Control IgG

Anti-AKAP13 antibody (ab99377)参考文献

ab99377 has not yet been referenced specifically in any publications.

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