Anti-AKAP13/p47抗体(ab99377)
Key features and details
- Rabbit polyclonal to AKAP13/p47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AKAP13/p47抗体
参阅全部 AKAP13/p47 一抗 -
描述
兔多克隆抗体to AKAP13/p47 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human AKAP13/p47 aa 2750-2850. NP_009131.2
Database link: Q12802 -
阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99377于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 308 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 308 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Isoform 6 stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions. -
组织特异性
Isoform 3 and isoform 6 are found in hematopoietic cells, skeletal muscle, lung, heart, estrogen-responsive reproductive tissues, including breast ductal epithelium. Also found in testis and breast cancer cell lines. Isoform 6 is not found in brain, placenta, liver, pancreas or kidney. Isoform 7 is expressed in myeloid and lymphoid lineages, a variety of epithelial tissues and skeletal muscle. Isoform 2 is predominantly found in the heart and at lower levels in the lung, placenta, kidney, pancreas, skeletal muscle and liver. -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger. -
结构域
Both the DH and PH domains are required for transforming activity. -
细胞定位
Cytoplasm; Cytoplasm. Nucleus and Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11214 Human
- Omim: 604686 Human
- SwissProt: Q12802 Human
- Unigene: 459211 Human
- Unigene: 710656 Human
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别名
- A kinase (PRKA) anchor protein 13 antibody
- A kinase anchor protein 13 antibody
- A kinase anchoring protein antibody
see all
图片
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All lanes : Anti-AKAP13/p47 antibody (ab99377) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 308 kDa
Exposure time: 10 seconds -
Lane 1 : Detection of Human AKAP13/p47 by Immunoprecipitation from HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded) using 3µg/mg lysate ab99377 lane 2 : Control IgG
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99377 被引用在 2 文献中.
- Liu Y et al. MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13. Front Oncol 11:754353 (2021). PubMed: 34722307
- Schrade K et al. An AKAP-Lbc-RhoA interaction inhibitor promotes the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells. PLoS One 13:e0191423 (2018). PubMed: 29373579