概述

  • 产品名称
    Anti-AKAP12抗体[JP74]
    参阅全部 AKAP12 一抗
  • 描述
    小鼠单克隆抗体[JP74] to AKAP12
  • 特异性
    This antibody recognizes Gravin (AKAP12) (approx. 300 kDa).
  • 经测试应用
    适用于: WB, IP, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Tissue/ cell preparation (Human) - fractionated membrane cortex proteins from neuron cell lines NT2-N and SK-N-BE(2).

  • 阳性对照
    • Rat brain cytosolic S1 fraction.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab198895 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab49849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.02 - 0.04 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 191 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

靶标

  • 功能
    Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • 组织特异性
    Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • 序列相似性
    Contains 3 AKAP domains.
  • 结构域
    Polybasic regions located between residues 266 and 557 are involved in binding PKC.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A kinase (PRKA) anchor protein (gravin) 12 antibody
    • A kinase Anchor Protein 12 antibody
    • A kinase anchor protein 250kDa antibody
    • A-kinase anchor protein 12 antibody
    • A-kinase anchor protein 250 kDa antibody
    • AKA12_HUMAN antibody
    • AKAP 12 antibody
    • AKAP 250 antibody
    • AKAP-12 antibody
    • Akap12 antibody
    • AKAP250 antibody
    • DKFZp686M0430 antibody
    • DKFZp686O0331 antibody
    • FLJ20945 antibody
    • FLJ97621 antibody
    • Gercelin antibody
    • Gravin antibody
    • Kinase scaffold protein gravin antibody
    • Myasthenia gravis autoantigen antibody
    • Myasthenia gravis autoantigen gravin antibody
    • OTTHUMP00000017426 antibody
    • OTTHUMP00000017427 antibody
    • Src-Suppressed C Kinase Substrate antibody
    • Srcs5 antibody
    • SSeCKS antibody
    • Tsga12 antibody
    see all

文献

This product has been referenced in:
  • Simon EP  et al. Transcriptomic characterization of fibrolamellar hepatocellular carcinoma. Proc Natl Acad Sci U S A 112:E5916-25 (2015). Read more (PubMed: 26489647) »
  • Radeva MY  et al. PKA compartmentalization via AKAP220 and AKAP12 contributes to endothelial barrier regulation. PLoS One 9:e106733 (2014). Human . Read more (PubMed: 25188285) »

See all 5 Publications for this product

客户评价及客户问答

Thank you for your patience.

I sincerely apologize about this delay, but I have good news:

Yes, rat is a tested species and I have updated our datasheet to reflect that. We therefore guarantee this antibodyto work with rat samples. ...

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Thank you for contacting us.

I have received the following information from the lab so far:

Wedid a Blast comparison of the rat and human proteins and although the overall identity was only 68%, there are long stretches of >90%...

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Thank you for contacting us.

The lab is looking into this and will let me know if there is a reason why rat was not listed as tested species. I will get back to you when I have more information.

Thank you for your patience and unde...

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Thank you for contacting us and for alerting us to the problem you are experiencing with our Anti-AKAP12 antibody ab49849. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for contacting Abcam. I have looked at the western blot image that you attached and I believe that the Akap12 band is actually the large band a the top of the image, not marked by a red arrow. If you would like my opinion of which band is the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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