, corresponding to C terminal amino acids 1769-1782 of Human AKAP12(Peptide available as
Jurkat and HeLa cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).Can be blocked with
Human AKAP12 peptide (ab23159).
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Contains 3 AKAP domains.
Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
Information by UniProt
A kinase (PRKA) anchor protein (gravin) 12 antibody
A kinase Anchor Protein 12 antibody
A kinase anchor protein 250kDa antibody
Western blot - Anti-AKAP12 antibody (ab9698)
All lanes :
Anti-AKAP12 antibody (ab9698) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (
Lane 2 : SW480 whole cell lysate ( ab3957) Lysates/proteins at 20 µg per lane. Secondary All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) ( ab65486) at 1/3000 dilution Performed under reducing conditions. Predicted band size: 196 kDa Observed band size: 268 kDa ( why is the actual band size different from the predicted?) Additional bands at: 100 kDa, 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 268 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12.
This product has been referenced in:
et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005).
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