- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-AKAP12抗体
参阅全部 AKAP12 一抗 -
描述山羊多克隆抗体to AKAP12
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宿主Goat
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide:
C-KQERESAKSELTES
, corresponding to C terminal amino acids 1769-1782 of Human AKAP12(Peptide available as ab23159.) -
阳性对照
- Jurkat and HeLa cell lysates
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
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Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab9698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).Can be blocked with Human AKAP12 peptide (ab23159). |
靶标
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功能Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
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组织特异性Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
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序列相似性Contains 3 AKAP domains.
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结构域Polybasic regions located between residues 266 and 557 are involved in binding PKC.
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
- Information by UniProt
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数据库链接
- Entrez Gene: 9590 Human
- Omim: 604698 Human
- SwissProt: Q02952 Human
- Unigene: 371240 Human
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别名
- A kinase (PRKA) anchor protein (gravin) 12 antibody
- A kinase Anchor Protein 12 antibody
- A kinase anchor protein 250kDa antibody
see all
图片
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Western blot - Anti-AKAP12 antibody (ab9698)All lanes : Anti-AKAP12 antibody (ab9698) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 :SW480 whole cell lysate (ab3957)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 196 kDa
Observed band size: 268 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 268 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12.
文献
This product has been referenced in:
- Lynch MJ et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005). Read more (PubMed: 16030021) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"