Anti-AKAP1抗体(ab69580)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to AKAP1
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from an internal region of human AKAP1.

  • 阳性对照
    • IHC-P: Human brain tissue WB: Extracts from HUVEC cells and COLO cells ICC/IF: COS7 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.
  • 组织特异性
    AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma.
  • 序列相似性
    Contains 1 KH domain.
    Contains 1 Tudor domain.
  • 结构域
    RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • 细胞定位
    Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A kinase anchor protein antibody
    • A-kinase anchor protein 1 antibody
    • A-kinase anchor protein 149 kDa antibody
    • AKAP 149 antibody
    • AKAP antibody
    • Akap1 antibody
    • AKAP1_HUMAN antibody
    • D-AKAP-1 antibody
    • DAKAP1 antibody
    • Dual specificity A-kinase-anchoring protein 1 antibody
    • mitochondrial antibody
    • PRKA1 antibody
    • Protein kinase A-anchoring protein 1 antibody
    • Protein kinase A1 antibody
    • S-AKAP84 antibody
    • SAKAP84 antibody
    • Spermatid A-kinase anchor protein 84 antibody
    see all

图片

  • All lanes : Anti-AKAP1 antibody (ab69580) at 1/500 dilution

    Lane 1 : extracts from COLO cells without immunising peptide
    Lane 2 : extracts from HUVEC cells without immunising peptide
    Lane 3 : extracts from COLO cells with immunising peptide

    Lysates/proteins at 30 µg per lane.


    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of AKAP1 in paraffin embedded human brain tissue using 1/50 ab69580. Left image: untreated sample. Right image: sample treated with immunising peptide.
  • Immunofluorescence analysis of AKAP1 in COS7 cells, using 1/500 ab69580. Left image: untreated cells. Right image: cells treated with immunising peptide.

文献

ab69580 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

The immunogen sequence is from 306aa to 345aa. Swissrot No.: Q92667.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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