概述

  • 产品名称Anti-Ajuba抗体
    参阅全部 Ajuba 一抗
  • 描述
    兔多克隆抗体to Ajuba
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ajuba.

    (Peptide available as ab93791.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; MEL-1; WI38.

性能

应用

Our Abpromise guarantee covers the use of ab64451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Ajuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
    • 细胞定位Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
    • 数据库链接
    • 别名
      • Ajuba LIM protein antibody
      • Ajuba, mouse, homolog of antibody
      • Jub ajuba homolog antibody
      • Jub antibody
      • LIM domain containing protein ajuba antibody
      • MGC15563 antibody
      • Protein ajuba antibody
      see all

    Anti-Ajuba antibody 图像

    • All lanes : Anti-Ajuba antibody (ab64451) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
      Lane 3 : WI-38 whole cell lysate (ab3960)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa
      Observed band size : 56 kDa
      Additional bands at : 23 kDa,48 kDa,73 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    Anti-Ajuba antibody (ab64451)参考文献

    This product has been referenced in:
    • Kalan S  et al. LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. Front Genet 4:95 (2013). Human . Read more (PubMed: 23755068) »

    See 1 Publication for this product

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