The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. The detection limit for ab125218 is 1ng/lane under non-reducing and reducing conditions.
Use a concentration of 1 µg/ml.
功能Required for assembly and stability of the aminoacyl-tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.
序列相似性Contains 1 GST C-terminal domain.
翻译后修饰Phosphorylated on serine residues in response to UV irradiation. Ubiquitinated by PARK2, leading to its degradation by the proteasome. Mutant PARK2 fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease.
细胞定位Cytoplasm > cytosol. Nucleus. Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus.
All lanes : Anti-AIMP2/p38 antibody (ab125218) at 1 µg/ml
Lane 1 : Rat liver tissue lysate Lane 2 : Rat lung tissue lysate Lane 3 : Rat kidney tissue lysate Lane 4 : Rat brain tissue lysate Lane 5 : Rabbit IgG (55kDa) Lane 6 : Jurkat whole cell lysate Lane 7 : CEM whole cell lysate Lane 8 : HUT whole cell lysate Lane 9 : U93T whole cell lysate