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Our Abpromise guarantee covers the use of ab93015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of fetal spleen tissue sections labeling AIM2 with ab93015 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence analysis of Jurkat cells labeling AIM2 with ab93015 at 1/200 dilution. Cells were fixed with methanol and permeabilized with citrate buffer, pH 6.0. The cells were fixed with 4% BSA for 30 minutes at 25°C, followed by incubation with Anti-AIM2 antibody (ab93015) in blocking buffer for 16 hours at 4°C. A goat-anti-rabbit FITC secondary antibody was used at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"