概述

  • 产品名称
  • 描述
    兔多克隆抗体to AIM2
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 93-341 of Human AIM2 (NM_004833).

  • 阳性对照
    • EC-109 and HepG2 cell lysates; Human fetal tonsil tissue

性能

应用

Our Abpromise guarantee covers the use of ab93015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-AIM2 antibody 图像

  • All lanes : Anti-AIM2 antibody (ab93015) at 1/500 dilution

    Lane 1 : EC-109 cell lysate
    Lane 2 : HepG2 cell lysate


    Predicted band size : 39 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of fetal spleen tissue sections labeling AIM2 with ab93015 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of Jurkat cells labeling AIM2 with ab93015 at 1/200 dilution. Cells were fixed with methanol and permeabilized with citrate buffer, pH 6.0. The cells were fixed with 4% BSA for 30 minutes at 25°C, followed by incubation with Anti-AIM2 antibody (ab93015) in blocking buffer for 16 hours at 4°C. A goat-anti-rabbit FITC secondary antibody was used at 1/100 dilution.

    See Abreview

  • Immunohistochemical staining of AIM2 in formalin fixed, paraffin embedded Human fetal tonsil showing nuclear staining with ab93015 at a dilution of 1/100.
  • ab93015 staining AIM2 in human monocytes (Apheresis) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab93015 at a 1/200 dilution for 2 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    See Abreview

Anti-AIM2 antibody (ab93015)参考文献

This product has been referenced in:
  • Zhen J  et al. AIM2 mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis by regulating caspase-1, IL-1ß, and IL-18. Mediators Inflamm 2014:190860 (2014). WB, IHC-P ; Human . Read more (PubMed: 24701032) »
  • Chattergoon MA  et al. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. PLoS Pathog 10:e1004082 (2014). Read more (PubMed: 24788318) »

See all 6 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Nov 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Loading amount
30 µg
Specification
skin
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Jurkat)
Specification
Jurkat
Fixative
Methanol
Permeabilization
Yes - citrate buffer, pH 6,0
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 29 2013

Thank you for your reply which has been forwarded to me as my colleague Anja is currently away from the office.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab93015 has been added to your...

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Thank you for your email.

I do understand and appreciate the amount of time your customer hasspent it the laboratory. Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in...

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Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 May 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Feb 01 2012

Thank you for contacting us.

I am sorry for the delay in gathering all the information that you have requested and getting back to you with it. I have contacted the suppliers of the products that you have enquired about and asked if the pe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 16 2012

Thank you for contacting us earlier, it was a pleasure speaking with you. I am sorry to hear that ab93015 is still giving you trouble.  I have set up a free of charge order for ab119845 for you, the order number is XXXXXX.  As I mentioned on the ph...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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