概述

  • 产品名称Anti-AIM2抗体
    参阅全部 AIM2 一抗
  • 描述
    兔多克隆抗体to AIM2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Horse
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Fetal lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab76423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 功能Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • 组织特异性Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • 序列相似性Belongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Absent in melanoma 2 antibody
    • AIM 2 antibody
    • Aim2 antibody
    • AIM2_HUMAN antibody
    • Interferon-inducible protein AIM2 antibody
    • OTTHUMP00000035296 antibody
    • PYHIN4 antibody
    see all

Anti-AIM2 antibody 图像

  • Anti-AIM2 antibody (ab76423) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-AIM2 antibody (ab76423) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-AIM2 antibody (ab76423)参考文献

This product has been referenced in:
  • Roy A  et al. Nuclear Innate Immune DNA Sensor IFI16 Is Degraded during Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus (KSHV): Role of IFI16 in Maintenance of KSHV Latency. J Virol 90:8822-41 (2016). Read more (PubMed: 27466416) »
  • Zhang X  et al. Cutting Edge: Ku70 Is a Novel Cytosolic DNA Sensor That Induces Type III Rather Than Type I IFN. J Immunol 186:4541-5 (2011). WB ; Human . Read more (PubMed: 21398614) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (B Cells)
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Loading amount 50 µg
Specification B Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2016

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. O...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"