概述

  • 产品名称
  • 描述
    兔多克隆抗体to AIFM3
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide (EGFSDRIVLC TLDRHLPYDR PKLSKSLDTQ PEQLALRPKE FFRAYGIEVL) corresponding to a region within internal sequence amino acids 216-265 of human AIFM3 (NP_653305).

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能
    Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential.
  • 组织特异性
    Ubiquitous. Expressed in bone marrow, cerebral cortex, liver, ovary, thymus, thyroid gland and tongue (at protein level).
  • 序列相似性
    Belongs to the FAD-dependent oxidoreductase family.
    Contains 1 Rieske domain.
  • 结构域
    The Rieske domain induces apoptosis.
  • 细胞定位
    Mitochondrion. Does not translocate to the nucleus upon induction of apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIFL antibody
    • Aifm3 antibody
    • AIFM3_HUMAN antibody
    • Apoptosis inducing factor 3 antibody
    • Apoptosis inducing factor like antibody
    • Apoptosis inducing factor like protein antibody
    • Apoptosis inducing factor, mitochondrion associated antibody
    • Apoptosis-inducing factor 3 antibody
    • Apoptosis-inducing factor-like protein antibody
    • FLJ30473 antibody
    • FLJ45137 antibody
    see all

Anti-AIFM3 antibody 图像

  • Anti-AIFM3 antibody (ab83791) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-AIFM3 antibody (ab83791)参考文献

ab83791 has not yet been referenced specifically in any publications.

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