Anti-AICDA抗体- ChIP Grade (ab59361)

概述

  • 产品名称Anti-AICDA抗体- ChIP Grade
    参阅全部 AICDA 一抗
  • 描述
    兔多克隆抗体to AICDA - ChIP Grade
  • 经测试应用适用于: ChIP, IHC-P, WB, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to 14 amino acids near the C-terminus of Human AID

  • 阳性对照
    • Ramos cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab59361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC Use a concentration of 10 µg/ml.

靶标

  • 功能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • 组织特异性Strongly expressed in lymph nodes and tonsils.
  • 疾病相关Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • 序列相似性Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activation induced cytidine deaminase antibody
    • Activation induced deaminase antibody
    • Activation-induced cytidine deaminase antibody
    • AICDA antibody
    • AICDA_HUMAN antibody
    • AID antibody
    • ARP 2 antibody
    • ARP2 antibody
    • CDA 2 antibody
    • CDA2 antibody
    • Cytidine aminohydrolase antibody
    • HIGM2 antibody
    • Integrated into Burkitt's lymphoma cell line Ramos antibody
    see all

Anti-AICDA antibody - ChIP Grade 图像

  • All lanes : Anti-AICDA antibody - ChIP Grade (ab59361) at 2 µg/ml

    Lane 1 : Ramos cell lysate
    Lane 2 : Ramos cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • ab59361 at 10µg/ml, staining human AID in Ramos cells
  • ab59361 (4 µg/ml) staining AICDA in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-AICDA antibody - ChIP Grade (ab59361)参考文献

This product has been referenced in:
  • Karpurapu M  et al. Krüppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation. PLoS One 9:e93362 (2014). WB, ChIP ; Mouse . Read more (PubMed: 24695324) »
  • Geisberger R  et al. Lysine residue at position 22 of the AID protein regulates its class switch activity. PLoS One 7:e30667 (2012). WB . Read more (PubMed: 22363466) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (B cell line)
Loading amount 30 µg
Specification B cell line
Gel Running Conditions Reduced Denaturing (14% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Dr. Yiming Zhong

Verified customer

提交于 Dec 20 2011

DISCOUNT CODE: XXXXXXXX Expiration date: [30/03/2012] I am very pleased to hear you would like to accept our offer and test ab59361 in Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please ...

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Thank you very much for your interest in ab59361. To our knowledge, ab59361 has not been tested in Mouse (predicted species only). Therefore, I can offer a discount off a future purchase if you buy ab59361 now, test it in Mouse and submit feedbac...

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Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (Splenic B lymphocytes)
Specification Splenic B lymphocytes
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Real-time PCR
Positive control Recruitment at Switch mu region
anti-H4 antibody ChIP
Negative control aid-/- cell extracts
Username

Abcam user community

Verified customer

提交于 Nov 24 2011

DISCOUNT CODE:XXXXXXXX Expiration date: <09-01-2012> I am very pleased to hear you would like to accept our offer and test ab59361 with "mouse". This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarant...

Read More

Thank you very much for your interest in ab59361. To our knowledge, ab59361 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab59361 now, test it in mouse and submit feedback to us in the form of a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Primary human B cell chromatin)
Specification Primary human B cell chromatin
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Taqman
Positive control IgM enhancer and switch region
Negative control epsilon globin and NeuroD1
Username

Abcam user community

Verified customer

提交于 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"