Anti-AICDA抗体- ChIP Grade (ab5197)


  • 产品名称Anti-AICDA抗体- ChIP Grade
    参阅全部 AICDA 一抗
  • 描述
    兔多克隆抗体to AICDA - ChIP Grade
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from residues 1 - 100 of Human AID.


  • 阳性对照
    • This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.




Our Abpromise guarantee covers the use of ab5197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).
ChIP Use at an assay dependent concentration.


  • 功能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • 组织特异性Strongly expressed in lymph nodes and tonsils.
  • 疾病相关Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • 序列相似性Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activation induced cytidine deaminase antibody
    • Activation induced deaminase antibody
    • Activation-induced cytidine deaminase antibody
    • AICDA antibody
    • AICDA_HUMAN antibody
    • AID antibody
    • ARP 2 antibody
    • ARP2 antibody
    • CDA 2 antibody
    • CDA2 antibody
    • Cytidine aminohydrolase antibody
    • HIGM2 antibody
    • Integrated into Burkitt's lymphoma cell line Ramos antibody
    see all

Anti-AICDA antibody - ChIP Grade 图像

  • Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

Anti-AICDA antibody - ChIP Grade (ab5197)参考文献

This product has been referenced in:
  • Dayal S  et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . Read more (PubMed: 21949728) »
  • Duquette ML  et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). Read more (PubMed: 15940261) »

See all 2 Publications for this product

Product Wall

Expiration date: February 13, 2013
Value: $337

This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab5197 in flow cytometry and include ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - other (Primary tonsillar B cells)
Specification Primary tonsillar B cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Taqman
Positive control AID ChIP followed by qPCR analysis for known AID binding sites:
IgG switch region
IgM switch region
BIRC3 promoter
Negative control AID ChIP followed by qPCR analysis for known sites where AID is known not to bind:
Neuro D1 gene

Dr. David Fear

Verified customer

提交于 Aug 13 2012

Thank you for contacting us this morning.

As discussed over the phone, I have generated a testing discount for anti-AICDA antibody (ab5197) for use in ChIP:

Discount code:

Expiration date:

This code wi...

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Thank you for the phone call and your patience.

The laboratory has now provided the images of the results with the new and old batch. The old batch has been tested by a collaborator and the new batch has been tested in the laboratory here - t...

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Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RW...

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