Anti-AICDA抗体(ab93596)

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概述

  • 产品名称
    Anti-AICDA抗体
    参阅全部 AICDA 一抗
  • 描述
    兔多克隆抗体to AICDA
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 16 amino acid peptide from near N terminal residues of Human AICDA

  • 阳性对照
    • Human placenta lysate, Human liver lysate and lymph node tissue

性能

应用

Our Abpromise guarantee covers the use of ab93596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
  • 组织特异性
    Strongly expressed in lymph nodes and tonsils.
  • 疾病相关
    Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
  • 序列相似性
    Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Activation induced cytidine deaminase antibody
      • Activation induced deaminase antibody
      • Activation-induced cytidine deaminase antibody
      • AICDA antibody
      • AICDA_HUMAN antibody
      • AID antibody
      • ARP 2 antibody
      • ARP2 antibody
      • CDA 2 antibody
      • CDA2 antibody
      • Cytidine aminohydrolase antibody
      • HIGM2 antibody
      • Integrated into Burkitt's lymphoma cell line Ramos antibody
      see all

    Anti-AICDA antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AICDA antibody (ab93596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AICDA antibody (ab93596)
      Immunohistochemical staining of formalin-fixed paraffin-embedded lymph node showing cytoplasmic staining with ab93596 at 1/100 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-AICDA antibody (ab93596)
      Immunocytochemistry/ Immunofluorescence - Anti-AICDA antibody (ab93596)
      ICC/IF image of ab93596 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93596, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-AICDA antibody (ab93596)参考文献

    ab93596 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab93596？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Dear,

    Can you please inform me about the price of ref AB93596?

    Thanks in advance,

    Thank you for your enquiry and your interest in our products.

    The price, the unit size, tested species, tested applications and other useful information can be found on the on-line datasheet.

    www.abcam.com/ab93596- Anti-AICDA antibo...

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    What the antibody show band of higher size?

    Thank you for contacting us.

    Unfortunately we do not have any other WB image than the published one. Although the observed band does appear like 50 kDa but we can confirm that the expected and observed band size would be 25kDa. We would be rem...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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