概述
- 产品名称Anti-AICDA抗体
参阅全部 AICDA 一抗 - 描述兔多克隆抗体to AICDA
- 经测试应用适用于: WB, IHC-P, ICC/IFmore details
- 种属反应性与反应: Mouse, Human
- 免疫原
A 16 amino acid peptide from near N terminal residues of Human AICDA
- 阳性对照
- Human placenta lysate, Human liver lysate and lymph node tissue
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - 纯化说明Affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab93596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/200 - 1/1000. Predicted molecular weight: 24 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
靶标
- 功能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
- 组织特异性Strongly expressed in lymph nodes and tonsils.
- 疾病相关Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
- 序列相似性Belongs to the cytidine and deoxycytidylate deaminase family.
- Information by UniProt
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数据库链接
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
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别名
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
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Anti-AICDA antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AICDA antibody (ab93596)Immunohistochemical staining of formalin-fixed paraffin-embedded lymph node showing cytoplasmic staining with ab93596 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-AICDA antibody (ab93596)ICC/IF image of ab93596 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93596, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-AICDA antibody (ab93596)参考文献
ab93596 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"