概述
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产品名称Anti-AHSG抗体
参阅全部 AHSG 一抗 -
描述兔多克隆抗体to AHSG
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宿主Rabbit
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经测试应用适用于: RIA, WB, ELISA, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Human Alpha 2 HS Glycoprotein
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS, pH 7.5 -
Concentration information loading... -
纯度Protein G purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab47979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| RIA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 40 kDa. | |
| ELISA | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
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组织特异性Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
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序列相似性Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
翻译后修饰Phosphorylation sites are present in the extracelllular medium.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
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别名
- 59 kDa bone sialic acid-containing protein antibody
- A2HS antibody
- Aa2-066 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-AHSG antibody (ab47979)ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
文献
ab47979 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"