概述
- 产品名称Anti-AHSG抗体
参阅全部 AHSG 一抗 - 描述兔多克隆抗体to AHSG
- 经测试应用适用于: RIA, WB, ELISA, ICC/IFmore details
- 种属反应性与反应: Human
- 免疫原
Human Alpha 2 HS Glycoprotein
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
- 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS, pH 7.5 -
Concentration information loading... - 纯度Protein G purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab47979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| RIA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 40 kDa. | |
| ELISA | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
- 功能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
- 组织特异性Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
- 序列相似性Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. - 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
- 细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
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别名
- 59 kDa bone sialic acid-containing protein antibody
- A2HS antibody
- Aa2-066 antibody
see all
Anti-AHSG antibody 图像
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Immunocytochemistry/ Immunofluorescence-Alpha 2 HS Glycoprotein antibody(ab47979)ICC/IF image of ab47979 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-AHSG antibody (ab47979)参考文献
ab47979 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"