Anti-AHNAK抗体[EM-09] (ab68556)

概述

  • 产品名称Anti-AHNAK抗体[EM-09]
    参阅全部 AHNAK 一抗
  • 描述
    小鼠单克隆抗体[EM-09] to AHNAK
  • 经测试应用适用于: WB, IP, ICC, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Rat
  • 免疫原

    Recombinant fragment corresponding to Human AHNAK (N terminal).

  • 阳性对照
    • HeLa human cervix carcinoma cell lysate and cells. Human primary fibroblasts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明ab68556 is purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号EM-09
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 629 kDa.
IP Use at an assay dependent concentration.
ICC 1/250. Permeabilization is required.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

靶标

Anti-AHNAK antibody [EM-09] 图像

  • Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
  • ab68556 at 1/250 dilution staining AHNAK (red) in human Hela cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).

  • Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/ml

    Performed under non-reducing conditions.

    Predicted band size : 629 kDa
    The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting: 30 mA, 90 min. Detection antibody concentration: 2 microgram/ml. Please note the conditions were not optimalized.
  • Ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).
  • Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-AHNAK antibody [EM-09] (ab68556)参考文献

ab68556 has not yet been referenced specifically in any publications.

Product Wall

We do sell normal mouse serum, the catalogue number is ab7486 and ab138705.

The affinity purified antibody we recommend is ab139582; http://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg-fab-pre-adsorbed-ab139582.html

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Thank you for following up.


I am happy to hear that the antibody is working as expected. Please do not hesitate to contact me if you have any additional questions. Good luck with your research!

Thank you for contacting Abcam regarding ab68556.


I have followed up with the lab and our shipping/receiving department and we have been unable to determine why this antibody is autofluorescing. I have confirmed that the antibody is not...

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Thank you for your patience. The lab sent the following explanation: The 150 kDa band seems to be a fragment arising due to manipulation with the extremely large AHNAK1 protein. Sometimes there are even more fragments observable, as the SDS PAGE ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (adipocytes)
Loading amount 30 µg
Specification adipocytes
Gel Running Conditions Non-reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"