概述

  • 产品名称Anti-AHI1抗体[M5]
    参阅全部 AHI1 一抗
  • 描述
    小鼠单克隆抗体[M5] to AHI1
  • 特异性ab93386 reacts with an epitope in the C-terminal region of Mouse AHI1 protein.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    A recombinant Mouse AHI1 with proprietary tag fusion protein.

  • 阳性对照
    • Baf3 cells and cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号M5
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明ICC/IF: 1/200 - 1/1000.
    IP: Use 1-2 µg / 200-800 ug protein.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 137 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Highly expressed in the most primitive normal hematopoietic cells. Expressed in brain, particularly in neurons that give rise to the crossing axons of the corticospinal tract and superior cerebellar peduncles. Expressed in kidney (renal collecting duct cells) (at protein level).
    • 疾病相关Defects in AHI1 are the cause of Joubert syndrome type 3 (JBTS3) [MIM:608629]. JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. JBTS3 shows minimal extra central nervous system involvement and appears not to be associated with renal dysfunction.
    • 序列相似性Contains 1 SH3 domain.
      Contains 7 WD repeats.
    • 细胞定位Cytoplasm > cytoskeleton > cilium basal body. Cell junction > adherens junction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abelson helper integration site 1 antibody
      • Abelson helper integration site 1 protein homolog antibody
      • Abelson helper integration site antibody
      • AHI 1 antibody
      • AHI-1 antibody
      • Ahi1 antibody
      • AHI1_HUMAN antibody
      • Contatins SH3 and WD40 domains antibody
      • dJ71N10.1 antibody
      • DKFZp686J1653 antibody
      • FLJ14023 antibody
      • FLJ20069 antibody
      • JBTS3 antibody
      • Jouberin antibody
      • ORF1 antibody
      • OTTHUMP00000017263 antibody
      • OTTHUMP00000017265 antibody
      • OTTHUMP00000234456 antibody
      • OTTHUMP00000234654 antibody
      • OTTHUMP00000234656 antibody
      see all

    Anti-AHI1 antibody [M5] 图像

    • ab93386 at 1/200 dilution staining AHI1 in 2% BSA-TBST by Immunofluorescence. Secondary antibody: 1/100 anti-mouse IgG in 2% BSA-TBST.
    • Western blot of Immunprecipitates using ab93386 at 2µg/800µg lysate.
      Lane 1: Parental BaF3 cells
      Lane 2: BaF3 + BCR-ABL (BaF3 cells transduced with BCR-ABL)
      Lane 3: BaF3 + BCR-ABL + ab93386 (M1, BaF3 cells transduced with both BCR-ABL and ab93386, (M1 is a clonal cell line))
      Lane 4: BaF3 + BCR-ABL + ab93386 (C2, BaF3 cells transduced with both BCR-ABL and ab93386, (C2 is a clonal cell line))

    Anti-AHI1 antibody [M5] (ab93386)参考文献

    This product has been referenced in:
    • Lee YL  et al. Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Mol Biol Cell 25:2919-33 (2014). Read more (PubMed: 25103236) »

    See 1 Publication for this product

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